Oct 07, 2014 introduction to high performance liquid chromatography and its parts. Hplc detectors types comparison principles pdf ppt. If so, share your ppt presentation slides online with. Now, coming to the chromatography then it is a technique which is availed by the scientist for separating. Highperformance liquid chromatography hplc scope instrumentation eluants, injectors, columns modes of hplc. If repeating recommendations from the published guideline, do not modify the recommendation wording. The hptlc validated method development for the quantification of. Ppt hptlc powerpoint presentation, free download id. The technique, which has become a standard analytical tool in. High performance thin layer chromatography hptlc and high. In chrome, your slideshare download is at the bottom of the browser window.
Jan 29, 2017 here in this article we provide hplc detectors types comparison principles pdf ppt. L syringe hamilton, bonaduz, switzerland which was programmed using. This utility allow you to download any presentation from slideshare. Click the ok button at the bottom of the prompt to finalize your choice. Nov, 2019 hptlc ppt free download automatically changes to flash or nonflash embed. Ppt video online download trematoda pixelmasterdesign. If you continue browsing the site, you agree to the use of cookies on this website. Apr, 2014 apr 26, 2020 chromatography ppt powerpoint presentation, semester, engineering chemistry class 11 notes edurev is made by best teachers of class 11. How to download ppt files from slideshare online for free.
You may not use any canadian cardiovascular society logos or trademarks on any slides or anywhere in your presentation or publications. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. This is the reason why product presentations must have the following. Hptlc take place in high speed capillary flow rang of the mobile phase, there are three main step in hptlc 1 sample to analyzed to chromatogram layer volume precision and suitable position are achieved by use of suitable instrument. The hplc detectors are used to detect the solute present in the eluent comes from the hplc column. Ppt video online download trematoda 20200428 20200428 tagged parasitology practical handbook lens optics staining.
Winner of the standing ovation award for best powerpoint templates from presentations magazine. An introduction to high performance liquid chromatography high performance liquid chromatography, or hplc, is the most common analytical separation tool and is used in many aspects of drug manufacture and research. Srivastava editor the present edited book is the presentation of 18 indepth national and interna. Ppt change in volume fraction of b solvent s constant f flow rate mlmin. Highperformance thin layer chromatography hptlc in the pharmaceutical industry. To keep for free in the future, please deactivate your adblocker or support this project by sending a small donation. Calendula officinalis, commonly known as marigold, has been traditionally used for its antiinflammatory effects. Highperformance thinlayer chromatography hptlc is an enhanced form of thinlayer chromatography tlc. Laboratory equipment united kingdom omicron research ltd. High performance thin layer chromatography by shaisej in types research. Introduction to high performance liquid chromatography and its parts. High pressure liquid chromatography 20 chem 4 introduction chromatography can be described as a mass transfer process involving adsorption using a nonpolar stationary phase and a mobile polar phase titrating through the column. The visual presentation of the complete chromatogram showing all samples and standards side by side is one of the most convincing arguments for highperformance thinlayer. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from.
High performance thin layer chromatography hptlc nc silica f254 10. Chapter1 introduction to analytical techniques and drugs. Free download revisiting defenses against large scale online password guessing attacks seminar ppt for cse and it paper presentation. Thinlayer chromatography, in analytical chemistry, technique for separating dissolved chemical substances by virtue of their differential migration over glass plates or plastic sheets coated with a thin layer of a finely ground adsorbent, such as silica gel or alumina, that is mixed with a binder such as starch or plaster of paris. Applications in forensic science presentation transcript.
At the end of each chapter there is a list of references andor further reading which will help the reader to develop their expertise in the technique. No other chromatographic technique can directly express the result as a colour image and make it available for. Chapter 1 2 3 introduction, chromatography theory, and. Presentations ppt, key, pdf logging in or signing up. Ppt video online download trematoda home ppt video online download trematoda. Thinlayer chromatography, in analytical chemistry, technique for separating dissolved chemical substances by virtue of their differential migration over glass plates or plastic sheets coated with a thin layer of a finely ground adsorbent, such as silica gel or alumina, that is mixed with a binder. Highperformance thin layer chromatography in the pharma industry.
Automated highthroughput elution instrument dbsms 500 to analyze dried blood spots in clinical diagnostics. The active component of the column, the sorbent or the stationary phase, is typically a granular. Introduces a new protocol called password guessing resistant protocol. An introduction to high performance liquid chromatography. A number of enhancements can be made to the basic method of thinlayer chromatography to automate the different steps, to increase the resolution achieved and to allow more accurate quantitative measurements. You can easily convert your pdf files to ppt with this online tool. A quality control for authentication of herbal phytochemicals. Download product specification sheet evaluation with wincats the visual presentation of the complete chromatogram showing all samples and standards side by side is one of the most convincing arguments for thinlayer chromatography. This edited book is the presentation of 18 indepth national and. Silica gel 65f modified highly purified silica gel upload from.
Normal phase adsorption tlc on silica gel with a less polar mobile phase, such as chloroform methanol, has been used for more than 90% of reported analysis. Loading slideshow in 5 seconds hptlc powerpoint presentation. Highperformance liquid chromatography hplc scope instrumentation eluants, injectors, columns modes of hplc partition chromatography adsorption chromatography ion chromatography size exclusion chromatography. Qualitative and quantitative analysis of unknown mixtures determining what is there, and how much. A number of enhancements can be made to the basic method of thinlayer chromatography to automate the different steps, to increase the resolution achieved and to. High performance thin layer chromatography hptlc and. Hptlc ppt free download automatically changes to flash or nonflash embed. The principle hptlc takes place in highspeed capillary flow range of the mobile phase. When you are preparing and designing the presentation, think. In addition to providing an introduction to hplc for pharmaceutical analysis it is intended that this book will be a useful resource. The 2 vertical lines of the h are the inflow arterial blood line that drains blood from the vascular access to the dialyser, and the outflow venous blood line that returns blood from the dialyser back to the vascular access 10. Ppt high performance thin layer chromatogarphyhptlc powerpoint presentation free to download id.
Furthermore, the online pdf converter offers many more features. Hptlc evaluates the entire chromatogram with a variety of parameters without time limits. Chromatography ppt powerpoint presentation, semester. Ppt thin layer chromatography tlc powerpoint presentation. Hptlc high performance thin layer chromatography ppt video. As you put up each slide, have in mind the key points you want to make with that slide one or two points. Hplc ppt introduction to high performance liquid chromatography. Apr 26, 2020 chromatography ppt powerpoint presentation, semester, engineering chemistry class 11 notes edurev is made by best teachers of class 11. High performance thin layer chromatogarphyhptlc is the property of its rightful owner. High performance thin layer chromatographyhptlc slideshare.
Thin layer chromatography tlc paper chromatography, electrophoresis anna drew with grateful acknowledgement for inspirational teaching received at a free powerpoint ppt presentation displayed as a flash slide show on id. Ppt high performance thin layer chromatogarphy hptlc powerpoint presentation free to download id. Applications in forensic science ppt download slideplayer. Prawez alam department of pharmacognosy college of pharmacy. The visual presentation of the complete chromatogram showing all samples and standards side by side is one of the most convincing arguments for thinlayer chromatography.
In the chrome browser, your slideshare presentation download appears at the bottom of the screen. Sample application is one imp and critical step for obtaining the good resolution for quantification by hptlc. A product presentation is very important as it leaves a first impression about the product that is to be launched. Professional imaging and documentation system for tlchptlc chromatograms and other planar objects with a stateoftheart digital ccd camera, connected by usb 3. This ppt is contributed by saikumar katam authorstream presentation. Prawez alam department of pharmacognosy college of pharmacy salman bin abdulaziz university. High performance thin layer chromatogarphyhptlc dr. How to give a paper presentation mit opencourseware. Hptlc is a fast separation technique and flexible enough to analyze a wide variety of samples. Chromatography equipment looks rather intimidating to anyone who has not handled it before, but on a closer look and as you get familiar with the equipment you realize that behind the network of wires, complex plumbing and circuitry is a simple machine with only a few major parts.
Useful data is provided throughout the book, such as. Different types of hplc detectors are given here for you for educational purpose. Few materials look homogenous but in real they are the combination of distinct substances. Laboratory equipment united kingdom omicron research. The causative agent of bovine trichomoniasis is a protozoan parasite names tritrichomonas foetus. Professional imaging and documentation system for tlc hptlc chromatograms and other planar objects with a stateoftheart digital ccd camera, connected by usb 3. Highperformance thinlayer chromatography wikipedia.
Evaluation with wincats the visual presentation of the complete chromatogram showing all samples and standards side by side is one of the most convincing arguments for thinlayer chromatography. Scribd is the worlds largest social reading and publishing site. Download as ppt, pdf, txt or read online from scribd. Hptlc high performance thin layer chromatography presentation transcript.
It is also known as planar chromatography or flatbed chromatography. Download 3 dimensional printing ppt presentation slides and seminar report in pdf for free for cse students. Hptlc plates provide improved resolution, higher detection sensitivity, and improved in situ quantification and are used for industrial pharmaceutical densitometric quantitative analysis. Here in this article we provide hplc detectors types comparison principles pdf ppt.
Ahd is carried out through h shaped ahd connections. Morphologically, it has a pearshaped body with three anterior flagella near the single large nucleus and one posterior free flagellu. No other chromatographic technique can directly express the result as a colour image and make it available for visual evaluation. This technique is advantageous in many ways as it is simple to handle and requires a short analysis time to analyze the complex or the crude sample. Samples were applied using a linomat 5 camag, muttenz, switzerland semiautomated applicator with a 100. Sample preparation for normal phase chromatography using silica gel alumina precoated plates, solvents non polar rp chromatography, usually polar solvents selection of chromatographic layer depends on the nature of material to be separated commonly used materials are silica gel 60f, alumina, cellulose etc. A very good example of such thing is the green plants as they have a mixture of distinct pigments.
The power of chromatography 9 comes from its ability to separate a mixture of compounds, or analytes, and. Hptlc ppt free download as powerpoint presentation. Free computer worms animation, download free clip art, free clip. Introduction of hptlc hptlc is the improved method of tlc which utilizes the conventional technique of tlc in more optimized way. For now, lets look at how to select and change the default colors for your presentation, so you dont have to change them manually each time you add a shape to your slides.